Open Access Presentation
Despite the beneficial effects of osteopathic manipulative techniques (OMT), there is a lack of in vitro models to understand the molecular mechanisms associated with these time-tested therapies. The Myofascial Release (MFR) technique is a non-invasive approach that involves passive stretching, hold and release, of the soft tissue to achieve myofascial homeostasis. Tissue-resident mesenchymal stem cells (MSC) can regulate the myofascial microenvironment by altering their secreted factors following stretch and release. Therefore, we initiated studies to develop an in vitro model to investigate the possible effects of stretch and release on MSC function, i.e. proliferation and differentiation capabilities, and changes in secreted factors. Preliminary optimization of protocols towards MSC culturing on a stretchable silicone membrane and their mechanical manipulation using the MechanoCulture FX (MCFX) machine, a programmable stretch and release device.
Smith, Ben; Notta, Shahn; and Mondal, Debasis, "Effect of stretch and release on myofascial stem cell function in vitro: A putative model to understand the molecular benefits of the myofascial release (MFR) technique" (2023). Research Day. 7.
Alternative and Complementary Medicine Commons, Biochemical Phenomena, Metabolism, and Nutrition Commons, Biological Phenomena, Cell Phenomena, and Immunity Commons, Chemical and Pharmacologic Phenomena Commons, Medical Cell Biology Commons, Medical Molecular Biology Commons, Osteopathic Medicine and Osteopathy Commons