Document Type

Presentation

Publication Date

Fall 10-5-2026

Abstract

Background: Lymphatic endothelial cells (LECs) are important for fluid homeostasis and inflammatory regulation, but excessive inflammation can disrupt lymphatic function. Creatine monohydrate (CrM) has potential anti-inflammatory properties, though its effects on inflammatory responses in LECs are not well understood. Objective: To determine whether CrM attenuates Tumor Necrosis Factor-alpha (TNF- α) induced inflammation in LECs by assessing Interleukin-6 (IL-6) and Nuclear factor kappa B (NFκB) expression. Methods: LECs were divided into four groups: control, CrM, TNF-α, and CrM + TNF-α. Treatment groups were exposed for 48 hours, with TNF-α and CrM administered simultaneously in the co-treatment group. IL-6 and NFκB protein and gene expression were assessed by Western blotting and qPCR. Group differences were analyzed by one-way ANOVA with Tukey’s and Dunnett’s post hoc tests. Results: TNF-α increased IL-6 protein expression versus control, both alone and with CrM (both p < .001), while CrM did not reduce IL-6 protein versus TNF-α alone (p = .912). IL-6 gene expression differed among groups (p = .014), with TNF-α increased versus control (p = .032); CrM + TNF-α did not differ significantly from TNF-α alone (p = .139). NFκB protein (p = .548) and gene expression (p = .304) did not differ significantly among groups. Conclusion: CrM did not significantly attenuate TNF-α-induced IL-6 expression, and no significant differences in total NFκB expression were detected.

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